bms ccr2 22  (MedChemExpress)

Journal: Communications Biology

Article Title: Aging promotes a RAGE-dependent increase in breast cancer metastasis

doi: 10.1038/s42003-026-10022-4

Figure Lengend Snippet: Transwell invasion assays using Py8119 ( A ) and 4T1 ( B ) breast cancer cells were performed using 1% serum pooled from three mice per group (young RAGE⁺/⁺, aged RAGE⁺/⁺, or aged RAGE −/− ) as the chemoattractant, and invasion was quantified after 24 h. Transwell assays assessing migration ( C ) and invasion ( D ) of Py8119 cells toward 1% serum pooled from aged RAGE⁺/⁺ mice ( n = 3) were performed in the presence of the RAGE inhibitor TTP488 (1 μM), the S100A8/9 inhibitor paquinimod (25 μM), the CCR1 inhibitor BX471 (10 μM), or the CCR2 inhibitor BMS-CCR2-22 (10 μM). Data represent mean ± SEM from four independent biological experiments. Statistical analysis: one-way ANOVA with Dunnett’s multiple-comparisons test. * p < 0.05; ** p < 0.01; *** p < 0.001; and **** p < 0.0001.

Article Snippet: Where indicated, inhibitors were added directly to both upper and lower chambers at the time of plating: 1 μM TTP488 (MedChemExpress) for RAGE inhibition, 25 μM Paquinimod (Cayman Chemicals) for S100A8/9 blockade, 10 μM BX471 (MedChemExpress) for CCR1 inhibition, or 10 μM BMS-CCR2-22 (MedChemExpress) for CCR2 inhibition.

Techniques: Migration

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on monocyte migration in vitro. ( A ) Schematic diagram of the Boyden chamber-based monocyte chemotaxis assay toward lung cell culture supernatant (LCCS). Monocytes in input cells and migrated cells were quantified by flow cytometry, and the migration efficiency (number of monocytes in migrated cells/number of monocytes in input cells [%]) was calculated. ( B ) Migration efficiency of monocytes after CCR2 inhibitor (BMS CCR2 22) pretreatment. n = 3 or 4 per group. ( C ) Concentrations of 13 chemokines (CXCL1, CXCL5, CXCL9, CXCL10, CXCL13, CCL2, CCL3, CCL4, CCL5, CCL11, CCL17, CCL20, and CCL22) as migration factors in the supernatant of lung tissue-derived cells of C57BL/6J mice after 72 h of culture. ( D ) Migration efficiency of monocytes pretreated with DSF, DDC, Cu(DDC) 2 , and cyanamide (ALDH inhibitor). n = 3 per group. ( E ) Percentage of viable bone marrow cells pretreated with DSF, DDC, Cu(DDC) 2 , and cyanamide (ALDH inhibitor). The percentage of viable cells not treated with inhibitors was used as the reference (100%). n = 3 per group. * P < 0.05, ** P < 0.01 (Tukey’s test).

Article Snippet: Thereafter, we incubated these cells at 25 °C for 90 min with or without various inhibitors such as CCR2 inhibitors BMS CCR2 22 (Tocris), DSF, DDC (Tokyo Chemical Industry), Cu(DDC) 2 (Tokyo Chemical Industry), and cyanamide (SIGMA), an ALDH inhibitor similar to DSF.

Techniques: Migration, In Vitro, Chemotaxis Assay, Cell Culture, Flow Cytometry, Derivative Assay